The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus. 相似文献
Ileal indigestible protein may be an important factor for the occurrence of post weaning diarrhoea in piglets. Depending on the quantity of indigested protein, an imbalance in the microbial community can occur, resulting in an overgrowth of pathogens. Diet viscosity may influence nutrient digestibility and substrate availability for microbial growth. Therefore, we examined the effect of dietary viscosity in piglet nutrition. Carboxymethylcellulose (CMC) was added as a model substance in order to increase diet viscosity. The effect of 1% supplemented CMC on nutrient digestibility, morphological and physiological parameters was measured in piglets 3 weeks post weaning at 26 days of age. The main effects of inclusion of CMC were:
– A 10% slower gastric emptying rate (P = 0.18).
– An increased protein hydrolysis in the gastric fraction, measured as increased nitrogen concentration in the liquid phase of gastric contents (26% in control vs. 34% in experimental group; P = 0.13).
– An increased production of the protein hydrolysing enzyme amino-peptidase from 359 U/g protein for the control diet to 516 U/g protein for the experimental diet (P < 0.01).
– A numerical increase in apparent ileal protein digestibility by 6% from 75.2% to 79.5% (P = 0.22) and a significant increase in faecal protein digestibility by 5% (P = 0.044).
These results indicate that an optimized diet viscosity might be one factor exerting a beneficial influence on protein digestibility. We hypothesize that the effect of diet viscosity on gastric emptying rate is the major factor. 相似文献
Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included. 相似文献